Summary: Noninvasive immunoimaging holds great potential for studying and stratifying disease as well as therapeutic efficacy.Radiolabeled single-domain antibody mother nature burger krusty burger fragments (i.e., nanobodies) are appealing probes for immune landscape profiling, as they display high stability, rapid targeting, and excellent specificity, while allowing extremely sensitive nuclear readouts.Here, we present a protocol for radiolabeling an anti-CD11b nanobody and studying its uptake in mice by a 2003 kia spectra catalytic converter combination of positron emission tomography imaging, ex vivo gamma counting, and autoradiography.
Our protocol is applicable to nanobodies against other antigens.For complete details on the use and execution of this protocol, please see Priem et al.(2020), Senders et al.(2019), or Rashidian et al.(2017).